水稻對(duì)惡苗病菌侵染響應(yīng)的蛋白質(zhì)組分析

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中圖分類(lèi)號(hào)：S511；Q816 文獻(xiàn)標(biāo)志碼：A 文章編號(hào)：1008-0864（2025）06-0126-10

ProteomeAnalysisofRiceResponsetoGibberellafujikuroi Infection

SHAO Lihua，LI Peng （Instituteof PlantProtection，HeilongjiangAcademyofLandReclamationSciences，Harbin15oo38，China）

Abstract： Bakanae disease，a common and highly harmful fungal disease of rice，seriously impacts rice yield.To clarifytheup-regulateddiferential proteins of high-resistance varieties inresponse to the bakanaepathogen，the TMT（tandem mass tag）quantitative proteomics technology was employed to compare the proteomes before and after infection with the bakanae pathogen，and the significantly differentially expressed proteins with P?0.05 and a fold change （FC）>1.2 or <0.83 were screened.The resultsshowed that 124up-regulatedand75 down-regulated differentially expressed proteins were screened.The 124 proteins with up-regulated expression were subjected to gene ontology（GO）enrichment analysis，and got 88 enrichment items，among which 4 items were significantly enriched， including 2 biological processes （response to stress and response to oxidative stress），2 molecular functions （peroxidase activityand hemebinding），which should be beneficial toremove peroxides in rice bud tissueand had a protectiveefect oncels.Atotalof45 pathways were obtainedby（kyoto encyclopediaof genes and genomes KEGG） enrichment analysis，and3 pathways were significantly enriched for upregulated differential proteins including phenylpropanoidbiosynthesis，thebiosynthesis of secondarymetabolitesand phenylalaninemetabolism， respectively，whichshould be beneficialtoimprove diseaseresistanceof rice.Therewere3differential proteins （Os01t0327400-01，Os12t0112000-01，Os01t0963000-04）were common among 4 itemsof GO significant enrichmentand3pathways ofKEGG significant enrichment，which couldbethe keyproteins inthebakanaedisease resistance.Above results provided scientific theoretical basis for the research of disease resistance.

KeyWords：rice；bakanae disease；Gibberella fujikuroi species complex；proteome；disease resistance

水稻惡苗病又名徒長(zhǎng)病，俗稱(chēng)公稻子，其病原菌有性態(tài)最初被命名為藤倉(cāng)赤霉菌[Gibberellafujikuroi（Sawada）Wollenw]，無(wú)性態(tài)命名為串珠鐮孢菌（Fusariummoniliforme），近年來(lái)將惡苗病菌有性態(tài)更名為藤倉(cāng)赤霉復(fù)合種（Gibberellafujikuroispeciescomplex，GFSC），該病是危害性強(qiáng)的水稻常見(jiàn)真菌性病害，廣泛分布于世界各個(gè)稻區(qū)，在水稻生育后期發(fā)病重的地塊發(fā)病率達(dá)10%＼～20%，嚴(yán)重影響水稻產(chǎn)量[1-2]

化學(xué)農(nóng)藥能有效殺死種子表面的病原菌或抑制其活動(dòng)，控制種子傳病，降低苗期發(fā)病率，最大限度地控制病害的再侵染，降低大田產(chǎn)量損失。（剩余8957字）