秋水仙素誘導(dǎo)陽光玫瑰葡萄多倍體的效果研究

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中圖分類號：S663.1 文獻標(biāo)志碼：A 文章編號：1002-2910（2025）02-0038-05

Study on the effects of colchicine-induced polyploidization in Shine Muscat grapes

ZHANGShihao， SUNXinru，F(xiàn)ENG Haonan，YAO Yuxin* （College of Horticulture Science and Engineering， Shandong Agricultural University， Tai'an，Shandong271018，China）

Abstract： The shoot segments of Shine Muscat grapes were used as materials， and 0 . 2 % 0 . 4 % ， 0 . 8 % ，and 1 . 2 % colchicine solutions were applied to treat the shoot with different times by the cotton immersion and soaking methods， the study was aimed to obtain the tetraploid grapes. The results showed that soaking grape branch segments in 0 . 4 % colchicine （ and 1 % dimethyl sulfoxide （ 2 0 0 m L ）for 6 days resulted in the greatest mutagenic effect， with a lethal rate of 5 0 . 0 0 % （20號 and a mutation rate of 6 . 6 0 % ， respectively. A total of 12 tetraploid and 2 mixed polyploid plants of the Sunshine Rose were obtained; The cotton soaking method with 1 . 2 % colchicine 2 0 0 m L + 1 % dimethyl sulfoxide 2 0 m L cotton soaked grape branch segments for 6 days showed the greatest mutagenic effect， with a lethal and mutation rate of 2 5 . 0 0 % and 6 . 0 0 % ， respectively.

Key words： grape; Shine Muscat; colchicine; induce; polyploidy

多倍體作物具有產(chǎn)量高、器官巨大、抗病抗逆能力強等特性，生產(chǎn)上有廣泛推廣應(yīng)用[1。（剩余6262字）