棉隆熏蒸對(duì)十字花科根腫病土壤微生物群落結(jié)構(gòu)影響

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關(guān)鍵詞：棉??；熏蒸；高通量測(cè)序技術(shù)；土壤微生物群落結(jié)構(gòu)；多樣性

中圖分類(lèi)號(hào)：S435.6 文獻(xiàn)標(biāo)志碼：A 文章編號(hào):2097-2172(2025)06-0572-07

doi:10.3969/j.issn.2097-2172.2025.06.014

Effects of Dazomet Fumigation on Soil Microbial Community Structure in Relation to Clubroot Disease of Cruciferous Crops

JING Zhuoqiong,XU Shengjun, Sun Qian, Guo Zhijie (Institute of Plant Protection, Gansu Academy of Agricultural Sciences,Lanzhou Gansu 73oo7o, China)

Abstract:Theefectsof dazometfumigationonthemicrobialcommunitystructureofcruciferous clubrotsoil were studied aimingtoprovideatheoreticalbasisfortheaplicationofdazometfumigationincontrolingsoil-boediseasesofcruciferous vegetables.High-throughputsequencing technologywasemployedtoanalyzethesoil microbialcommunitystructureafterdazomet fumigationinclubroot-infectedsoil.Resultsshowedthattheabundanceoffungiandthediversityofbacteriainthesoilwere significantlyreducedbysoilfumigation,andtheOUTs(operationaltaxonomicunit)clusteringresultsofbacteriaandfugiby β diversityanalysisshowedthatthfumigationsignificantlychangedthestructureofmicrobialcommunitiesinthesoil.Thecommunity structuresimilarityusingtwodiferentconcentrationsoffumigationtreatmentwashigh,butshowedlowsimilaritytountreatedand water-treatedcontrolsamplescolltedonthedayofmembraneremoal.Inadition,teuseofdiferentconcentrationsoffuigation (375,400kg/ha)couldalsoincreasetheabundanceofbeneficialfungisuchasChaetomiumandTetracladium,andreducethe abundanceofpathogenicfungisuchasAlternariaandFusarium.However,fumigationalsohadanegativeimpactoncertain beneficial soil fungi.The highest increase inabundance of Chaetomium was 6.69% ,and the highest decrease in Alternaria was 8.84% ：

Key Words:Dazomet; Fumigation; High-throughputsequencing technology; Soil microbial community structure; Diversit

蔬菜產(chǎn)業(yè)是蘭州鄉(xiāng)村振興的支柱產(chǎn)業(yè)，蘭州市僅2023年蔬菜產(chǎn)量就達(dá)223.7萬(wàn)t，產(chǎn)值124億元，其中十字花科蔬菜是蘭州蔬菜產(chǎn)業(yè)的主栽蔬菜，榆中大白菜、甘藍(lán)、花椰菜等有一定優(yōu)勢(shì)的十字花科蔬菜已被認(rèn)證為全國(guó)地理標(biāo)志產(chǎn)品[]，是甘肅高原夏菜的主導(dǎo)蔬菜種類(lèi)[2-3]。（剩余11147字）