生防菌株YM3-3的鑒定和抑菌能力測定及發(fā)酵條件優(yōu)化

打開文本圖片集

中圖分類號：Q939.9；S513 文獻標志碼：A 文章編號：2097-2172（2025）04-0365-08

doi：10.3969/j.issn.2097-2172.2025.04.014

Identification， Antagonistic AbilityEvaluation， and Fermentation Optimization ofBiocontrol Strain YM3-3

WANG Chunming1， GUO Cheng1， ZHOU Tianwang1， HONG Liu’， XU Shenghai ， XU Yongfeng （1.Institute of Plant Protection，Gansu Academy of Agricultural Sciences，Lanzhou Gansu 73oo7o， China； 2.Wuwei Agricultural Technology Extension Centre，Wuwei Gansu 733Ooo， China； 3. Zhangye Plant Protection Station， Zhangye Gansu 734Ooo， China）

Abstract：To providebiocontrol strainresources for high-eficiencyandsafe microbial pesticides，theendophytic Bacilus subtilisY3-3，strainithidaliocontrolotentialsolatefromaizlaveasdentifidndtermentationondisee optimized.Thisstudyprovidedtechnicalparametersandatheoreticalbasisoncontrolmaizediseasesasbiocontrolagents.The inhibitionfstoicEoe melonis，V andFusariumoxysporumf.sp.vesinfectum.）wasmeasuredbytheduralculture methodTheresultsshowed that ithadgood antagonistic effect，and the inhibition rate against 1O pathogenic fungi was （53.19±3.78） % to （81.94±1.54） % ，showing good biocontrol potential.Acdigtooicalndeealys，sdntfdilustiId optimizethefermentationconditions，theoptimizationexperimentofcultureconditions wascariedout.Theresultsshowedthatthe optimalulturete，mpeature，atioalspeed，p，ndlqdvomeer3d27C，5Orin7nd9Oespectielye optimal combination of fermentation medium was15 g/L mannitol， 2 . 5 g / L peptone，7.5 g/L potassium nitrate，and 5 . 0 g / L calcium carbonate.

KeyWords：Bacilussubtilis；YM3-3；Antagonisticability；Fermentationcondition； Orthogonal test； Culturecondition； ）ptimization

自前，我國農(nóng)作物病蟲害防治主要以化學農(nóng)藥為主[1-2]。（剩余9990字）