感離蠕孢葉斑病多花黑麥草葉際微生物群落結(jié)構(gòu)與多樣性分析

打開文本圖片集

中圖分類號：S435.4 文獻標識碼：A 文章編號：1007-0435（2025）08-2423-1

Abstract：To investigate the changes of the structure and diversity of the phyllosphere microbial community after infection of Italian ryegrass（Lolium multiflorum） with Bipolaris sorokiniana，this study was conducted by artificially inoculating Italian ryegrass with B . sorokiniana spore suspension and collecting leaves of infected and healthy Italian ryegrass. High-throughput sequencing was used to investigate diferences in the structure and diversity of the phyllosphere microbial community.Results showed that there were significant differences in phyllosphere microbial community structure，and the species richness and diversity of phyllsphere fungi and phyllosphere bacteria were higher in infected leaves than in healthy leaves.The dominant bacterial phylum was Proteobacteria and the dominant fungal phylum was Ascomycota.Functional prediction revealed a significant increase in potential pathogenic phenotypes following the infection of Italian ryegrass，with the Proteobacteria contributing substantially to the pathogenic phenotypes.Differences in abundance of Proteobacteria across groups were consistent with trends in pathogenicity phenotypes.This study provides a theoretical basis for the mechanism of plant phyllosphere microbial community responses to pathogen infection.

Key words：Lolium multiflorum; Bipolaris sorokiniana; Phylosphere microbial community;Diversity;Highthroughput Sequencing

多花黑麥草（Loliummultiflorum）是一種高產(chǎn)優(yōu)質(zhì)牧草和草坪草，在世界各地廣泛種植，具有較高的環(huán)保價值和經(jīng)濟價值，隨著我國西南地區(qū)黑麥草的廣泛種植，其病害的發(fā)生對多花黑麥草的生產(chǎn)和利用造成較大經(jīng)濟損失[1-3]。（剩余16163字）