柱花草響應(yīng)炭疽菌和低磷脅迫的LysM-RLKs成員鑒定及表達(dá)模式分析

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Abstract：To identify the members of the LysM-RLKs family of Stylosanthes guianensis （Stylo） in response to Coletotrichum gloeosporioides and low phosphorus stress，we used transcriptomedata inoculated with Colletot richum gloeosporioides and treated with low phosphorus to identify and name LysM-RLKs family members， and predict their subcellular location and protein sequence by bioinformatics methods. The specificity of tissue expression and the expression pattrns in response to chitin，anthrax and low phosphorus treatment were analyzed through the related tests of Stylosanthes plants.The results showed that 1l genes，members of LysMRLKs family，were characterized by using the corresponding transcriptome data， namely S g L Y K I ， S g L Y K 2 a S g L Y K 2 b ， S g L Y K 3 a ， S g L Y K 3 b ， S g L Y K 4 a ， S g L Y K 4 b ， S g L Y K 5 ， S g L Y K 7 ， S g L Y K 8 and S g N F R 5 ： The prediction of protein localization showed that most of LysM-RLK proteins were localized on the plasma membrane，contained typical extracellular LysM domains，transmembrane domains and intracellular kinase domains.The prediction of protein structure showed that alpha helix and random coil were the major secondary structure of the proteins，and the LysM-RLK proteins were diferent in terms of the three-dimensional structure.The gene expression pattern analysis showed that all the identified LysM-RLKs members had tissue-specific expression pattern. The S g L Y K I ， S g L Y K 4 a ， S g L Y K 5 ， S g L Y K 7 and S g L Y K 8 may play important roles in responding to C ：gloeosporioides infection and chitin-induced immune responses，while S g L Y K 3 a may be involved in the responses to low phosphorus stress.This study could provide candidate genes for the breeding of anthracnose- and low phosphorus-resistant cultivars of stylo.

Key words： Stylosanthes guianensis； LysM-RLK family； Anthracnose； Low phosphorus stress； Expression patterns

植物模式識(shí)別受體（Pattern recognition receptor，PRR）主要有兩類蛋白：類受體樣激酶（Receptor-likekinases，RLKs）和類受體蛋白（Receptor-likeproteins，RLPs）。（剩余20213字）