CRISPR-Cas介導(dǎo)基因編輯技術(shù)的發(fā)展趨勢及研究進(jìn)展

摘要：CRISPR-Cas系統(tǒng)為細(xì)菌與古生菌的一種免疫防御機(jī)制?；谠撓到y(tǒng)的基因編輯技術(shù)在基因工程領(lǐng)域得到廣泛應(yīng)用，極大地加快了該領(lǐng)域研究的進(jìn)步。本文從CRISPR-Cas系統(tǒng)介導(dǎo)的基因組編輯技術(shù)的發(fā)展趨勢和最新應(yīng)用及現(xiàn)狀進(jìn)行綜述。

關(guān)鍵詞：CRISPR-Cas系統(tǒng) ; Cas9 ; 基因組編輯技術(shù)

中圖分類號：Q78 文獻(xiàn)標(biāo)識碼：A 文章編號：2096-3718.2022.06.0.06

Development tendency and research progress of CRISPR-CAS-based Genome engineering

YANG Yongchang， CHANG Shuai， ZHAO Xinyu

（Department of Clinical Laboratory， Seventh Medical Center of Chinese PLA General Hospital， Beijing 100010， China ）

Abstract： CRISPR-Cas system is used to resist exogenous viruses or plasmids in bacteria and archaea Acquired immune system of DNA invasion. The gene editing technology based on this system has brought a breakthrough revolution to the regulation and application of targeted genome modification. In this paper， we review the development and application of CRISPR-Cas system mediated genome editing technology， and provide reference for related researchers.

Keywords： CRISPR-CAS system; Cas9; Genome engineering

基因組編輯技術(shù)的迅猛發(fā)展引起科學(xué)界的廣泛重視。（剩余16433字）