虎杖在急性痛風(fēng)性關(guān)節(jié)炎大鼠中的入血成分及代謝產(chǎn)物分析

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中圖分類號(hào)R965；R917 文獻(xiàn)標(biāo)志碼A 文章編號(hào) 1001-0408（2025）13-1581-06

DOI 10.6039/j.issn.1001-0408.2025.13.05

Analysisofcomponentsmigrating to bloodandmetabolitesof Polygonumcuspidatum inrats with acute goutyarthritis

KE Caiyi，SHEN Meng，JI Li， WANG Xuechun，ZHU Yuqing，CHEN Xi， WANG Chengweiqi，MA Qun（School of Chinese Materia Medica，Beijing University of Chinese Medicine，Beijing 102488）

ABSTRACTOBJECTIVEToanalyze thecomponents migrating tobloodand metabolitesofPolygomumcuspidatum inrats with acute gouty arthritis （AGA）.METHODs SD rats were randomly divided into blank group，model group and P. cuspidatum group （ |10g/kg ，byraw material），with 6rats in each group.Except for blank group，AGA model was induced in the remaining groups byinjecting potassiumoxonateand sodiumurate；meanwhile，theywereadministeredcoresponding drugsolutionsorwater intragastrically，onceaday，for1Oconsecutive days.Thehistopathological morphologyof thekne jointtisses inratswas observed;rat serum samples were collected，and the components migrating to blood and metabolites of P. cuspidatum were analyzed byusing UPLC-Q-Exactive-Orbitrap-MS. RESULTS Following the intervention with P. cuspidatum，the histopathological morphologyoftheknee jointsynovialtisueinAGAratsshowedsignificantimprovement，withreduced inflammatorycel infiltrationandhyerplasiandthepreservationofteoneyombikestructureintegrityInbothpositiveandnegativeiodes， a total of 67 chemical components were detected in the serum of rats from P. cuspidatum group，including 25 prototype components and42metabolites.The involvedcompoundtypes encompassed stilbenes，anthraquinones，naphthols，andflavonoids，among others.Temetabolicreactionsidentifiedincludedmethylation，acetylation，sulfation，andglucuronidationNotably，compounds suchas polydatin，resveratrolandemodinwerecapableofentering thebloodstreamintheirprototypeformsandundergoingin vivo metabolism.CONCLUSIONsCompoundssuch aspolydatin，resveratrol and emodinarelikelytobe theactivecomponents responsible for the anti-AGA effects of P.cuspidatum.

KEYWORDsPolygomumcuspidatum；acutegoutyarthritis；componentsmigrating toblood；metabolites；pharmacokinetics; active components；UPLC-Q-Exactive-Orbitrap-MS

急性痛風(fēng)性關(guān)節(jié)炎（acutegoutyarthritis，AGA）是由尿酸鈉結(jié)晶沉積引發(fā)的急性關(guān)節(jié)炎疾病，在全球患病率呈逐年上升趨勢，成年人群患病率已達(dá) 0.68%～3.90%t 11。（剩余10473字）