鮑姆桑黃 (Sanghuangporus baumii)HSP20基因家族的分子特征及不同發(fā)育階段的表達模式分析

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中圖分類號：Q931 文獻標識碼：A DOI編碼：10.3969/j.issn.1006—6500.2025.05.001·

Molecular Characteristics and Expresson Patterns of the HSP20 Gene Family in Sanghuangporus baumi at Different Developmental Stages

IAN Yanting，QI Xin，LIU Hao，LIAO Fangzhou，TONG Yana （TheResearch InstituteofForestryandPomology，TianjinAcademyofAgricultural Sciences，Tianjin3Oo384，China） Abstract：Toelucidatethebiologicalfunctionsandmolecularmechanismsof theheatshockprotein2O（HSP20）genefamilyinthe medicinalfungusSnghuangporusbaumi，thisstudysystematicallyidentifiedtheSbHSP2Ofamilymembersbasedonholeenome dataandinvestigatedtheirrolesinfungalgrowthregulation.Theresultsdemonstratedthat11SbHSP2Ogeneswereidentifiedin S baumi，ncodingproteisithmolecularweightsrangingfrom17.5to8.96Daandisoelectricpoits（p）distrbutedbtwen5.07 and10.38.Phylogeneticanalysisrevealed highhomologybetweenSbHSP2Oproteinsand HSP2OproteinsfromPleurotusostreatus （average sequence identity： 76.8 % ）.The promoter regions harbored 12 classes of stress-responsive cis acting elements，including ABRE，MBS，ndSRE，withtheSH2-7promoterontaningsevendistictstresselatedelements.Expressonprfligaaly sisrevealed thatthe transcription levelsof SbHSP2O-2and SbHSP20-IO wererelativelyhighinmyceliacultured for5days，with valuesof 23.20and9.023，respectively.Meanwhile，theexpresionlevelsofSHSP2-6，SHSP2-7，andSHSP20-10imyelia culturedfor9dayswere52，6.09，nd98，espectively.Colinarityalysisucoveredcromosomalhomologousgiosb tweenS.baumiiandAgaricusbisporus.Inonclusion，thisstudyprovidesthefirstsystematiccharacterzationofthemoleularfeaturesand expression regulation of the SbHSP2O gene family in S .baumii.The findings highlightSbHsP2O-1Oasakeycandidate geneforenhancingfungalstressresistance，oferingboththeoreticalfoundationsandgeneticresourcesformolecularbreedigof stress-tolerant medicinal fungi.

Key words： small heat shock protein （HSP2O）; gene structure; phylogeny; chromosomal localization

鮑姆桑黃（Sanghuangporusbaumii）是一種珍貴的藥用真菌（此前被歸類為鮑氏針層孔菌和鮑氏木層孔菌），幾個世紀以來，在中國、韓國、日本和其他一些亞洲國家被用作傳統(tǒng)草藥。（剩余9144字）