光譜流式細(xì)胞儀在生物材料檢測(cè)中的應(yīng)用研究

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中圖分類號(hào)：Q631 文獻(xiàn)標(biāo)志碼：A DOI：10.3969/j.issn.1007-7146.2025.03.004

Abstract：Asadetection instrument developed rapidlyinrecent years，the spectral flowcytometer primarilycomplemented traditionalflowcytometry.Thisarticledemonstratedtheperformanceof threespectralflowcytometers inthedetectionof fluorescent materials，demonstratingtheirpotentialapplications inthefieldofbiological materials.Studies showedthatthe spectral flowcytometercouldnotonlyexplorefluorescencefromnon-primarybandsofknown fluorescent dyes，but also decompose overlapping spectrabetween fluorescentmaterials，andreagentkitdetectionchannels，esulting inmoreaccurate analyticalresultsdditioallyituldtecttesctralsiftsoffuorestomaterialsiinell.Ialltstral flowcytometercouldrecordthespectralcharacteristicsoffluorescentmaterialsenteringcels，analyethespectralbehaviorof fluorophores within cells，and spectrallyresolve fluorescence signals that overlapped with detection channels，thus providing objective fluorescence data.These capabilities opened up new paths for the research offluorescent materials，providing theoreticalfoundationsaswellaspracticalreferencesforthewidespreaduseofspectralflowcyometryinbiomaterialsscience

Key words： spectral flow cytometry; biological materials; fluorescent materials; fluorescence spectrum shift;spectral separation

（ActaLaser BiologySinica，2025，34（3）：221-228）

近年來(lái)，流式細(xì)胞儀及流式細(xì)胞術(shù)發(fā)展迅猛，研究人員創(chuàng)新研究了多種類型的流式細(xì)胞儀，包括光譜流式細(xì)胞儀（spectrumflowcytometry）、質(zhì)譜流式細(xì)胞儀（masscytometry）、成像流式細(xì)胞儀（imagingcytometry）以及組織成像質(zhì)譜流式細(xì)胞儀（imagingmasscytometry）等。（剩余12814字）