海水葉綠素a傳感器熒光性能評估方法

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A standardized method for evaluating the fluorescence performance of

seawater chlorophyll-a sensors

WANG Aijun，WANG Cong， SHI Chaoying， ZHAO Hong （Technology Innovation Center for Marine Metrology and Instruments Test， MNR，National Center of Ocean Standards and Metrology，Tianjin ，China）

Abstract：Seawater chlorophyl-asensorsare essential tools for marineecological monitoring，enabling thedetectionof spatialand temporal variations inchlorophylla concentration.However，thesesensorsaresusceptible to measurement drift，whichcancompromisedatareliability.Thisstudyproposesametrologicalapproachforevaluating sensor performance using fluorescein sodiumasa reference standard.Sensor performance was assssed in terms of linear responserange，accuracy，precision，and stabityResults indicatedastrong positive corelationbetween fluorescence intensityandfluoresceinconcentration.Basedonthefittedcalibrationcurve，indicationerorand standarddeviation were calculated.The linearresponserange wasdeterminedbycontroling thecorrelationcoeficient，whereasstability was

assessed through repeated measurements over diffrent time periods.Within the linear range of 0 to 200μg/L ，the maximum measurement error was ?2.00μg/L ，and the relative standard deviation was <0.20% .The sensor exhibited consistentperformance from2021to2022.Maintaining consistent pipetingaccuracywasidentifiedasacriticalfactorfor ensuringmeasurement reliability.

Key words ： seawater chlorophyll-a senaor; fluorescence standard material； evaluation method； pipete accuracy

海洋中葉綠素a質(zhì)量濃度及其時間變化可以反映海水中浮游植物的豐度及其變化規(guī)律，在一定程度上反映水體中藻類數(shù)量和水質(zhì)狀況[1-2]，是海洋生態(tài)監(jiān)測的常規(guī)監(jiān)測項目之一，在研究浮游植物群落、海洋生態(tài)環(huán)境監(jiān)測、赤潮防災(zāi)減災(zāi)等方面起著重要的作用。（剩余6498字）