歐洲小葉椴體細胞胚胎發(fā)生及植株再生體系建立

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中圖分類號：S722;S792.36 文獻標志碼：A 文章編號：1000-2006（2025）04-0079-09

Plant regeneration through somatic embryogenesis in Tilia cordata

LI Yin12，YU Sinong3，YAN Lingjun2，WANG Huanli2*，TANG Shijie 1，2 *

（1.Nanjing Universityof ChineseMedicine，Nanjing 21OO23，China；2.Instituteof Botany，Jiangsu Provinceand Chinese Academy of Sciences （Nanjing Botanical Garden Mem.Sun Yat-Sen），Jiangsu Key Laboratory for Conservation andUtilizationof Plant Resources，Nanjing 210014，China；3.Yancheng Forest Farm，Yancheng 224136，China）

Abstract：【Objective】Thisstudyaims todevelopacompletesomaticembryogenesissystem forTiliacordataby systematicallyoptimizing theculture medium composition to identifyoptimal conditions for somaticembryo inductionand subsequent plant regeneration.【Method】Immature zygotic embryos of T cordata wereused asexplants.The effects of diffrentconcentrationsandcombinationsofplantgrowthregulators2，4-dichlorophenoxyaceticacid（2，4-Dand 6-benzylaminopurine（6-BA）oncallusinduction，proliferation，and somatic embryo induction eficiency were systematicallyinvestigated.Additionally，theimpactsofdiferentbasalmedia，indole-3-butyricacid（IBA） concentrations，andactivated charcoal suppementationon somaticembryogerminationand plant regeneration were evaluated.【Result】Optimal callus induction was achieved at a concentration of 3.O mg/L 2，4 -D combined with 0.2 mg/L6-BA，yielding an induction efficiency of 64.25% .Subsequent proliferation of embryogenic callus demonstrated maximal growth potential under identicalhormonal conditions （3.0mg/L2，4-D+O.2 mg/L 6-BA），as evidenced byan exceptional proliferation coefficient of 1063.72% .Forsomatic embryo induction，the same hormonal formulation（3.0 mg/L 2，4-D+0.2 mg/L 6-BA） proved most effective，generating somatic embryos at a frequency of 46.41% . Complete plantregenerationwassuccesfullyaccomplished throughtransfering of developed somatic embryos toMSbasal medium 收稿日期Received：2024-11-20 修回日期Accepted：2025-03-12 supplemented with 0.5mg/L IBA in the absence of activated charcoal，with all regenerated plants exhibiting normal morphological characteristics.【Conclusion】In this study，a complete somatic embryogenesis system of T cordata was initiallyestablished，providingabasis forsubsequentestablishmentofaneficientpropagationandgenetictransformation system for Tilia.

Keywords：Tilia cordata；embryogenic callus；somatic embryo；somatic embryo regeneratio

歐洲小葉椴（Tiliacordata）屬于錦葵科（Malvaceae）椴樹屬（Tilia），為落葉喬木，原產(chǎn)自歐洲，具有良好的觀賞價值和較強的環(huán)境適應性。（剩余17741字）