丹參酮IA調控PKM2/EGFR通路抑制平滑肌細胞增殖和代謝重編程的研究

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[中圖分類號］R285.6 [文獻標志碼］A [文章編號]0256-7415（2025）10-0171-09

DOl：10.13457/j.cnki.jncm.2025.10.033

Study on Tanshinone IA in Inhibiting Proliferation of Smooth Muscle Cells and Metabolic Reprogramming by Regulating PKM2/EGFR Signaling Pathway

HUANG Feiyan1，WU Shuling2，ZHONG Zhiqiang1

1.Medical ClinicalLaboratory，TheFirstAfiliatedHospitalofZhejiangChineseMedicalUniversityZhejiangProvincialHospitalf TraditionalChineseMedicine，HangzouZhejiang306，China；2.DeparmentofGeritrics，TheFirstAfiliatedHospitalofZejang Chinese Medical University/Zhejiang Provincial Hospitalof Traditional Chinese Medicine，Hangzhou Zhejiang 310o06，China Abstract：Objective：To explore the molecular mechanismof Tanshinone IIA in inhibiting oxidized low-density lipoprotein（ox-LDL） induced proliferationof vascularsmooth musclecelsand Warburg efect byregulating the PKM2/ EGFR signaling pathway.Methods：The human aortic smooth muscle cels were used as the research object.The negative control group， the ox-LDL control group and the low and high doses of Tanshinone 11Agroup（10，50μg/mL） 0 wererespectivelysetup；MTTassy was usedto detect the efectof Tanshinone IA interventionon the proliferationof smooth muscle cels induced byox-LDL，and flow cytometry was used to detect the changes in cell cycle；the changesin protein expresion of pyruvate kinase isozyme type M2（PKM2），phosphorylated epidermal growth factor receptor（pEGFR），active β- catenin，cyclin D1，Glut1 and LDHA protein was detected by Western blotting， and the levels of PKM2 mRNA were detected by fluorescence quantitative PCR；glucose consumption and lactic acid production were measured by spectrophotometry.Results：Tanshinone IIA inhibited the ox-LDL-induced cel proliferation inadose-dependent manner.The proportionof cels in G1 phase was elevated，andreduced in S phase and G2/M phase.TanshinoneIIA inhibited the expression ofPKM2by the regulationof proteins and transcriptional level， down-regulated the protein expression of pEGFR，active β- catenin，cyclin D1，Glut1 and LDHA，and reduced glucose uptake and lactic acid production. Conclusion： Tanshinone IA can inhibit the ox-LDL-induced proliferation of smooth muscle cells and metabolic reprogramming by regulating PKM2-mediated EGFR.

Keywords：Tanshinone IA；Vascular smooth muscle cels；oxidized low-density lipoprotein；Pyruvate kinase isozyme type M2； Phosphorylated epidermal growth factor receptor； Metabolic reprogramming

動脈粥樣硬化（AS）可累及心、腦、腎、眼等多器官及外周血管系統(tǒng)，是泛血管疾病的主要病理基礎。（剩余11342字）