高效液相色譜法測定砂生槐種子中3種生物堿含量的方法研究

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Studyon the determinationof three alkaloids in the fruit of Sophoraammosa by high-performance liquid chromatographyHouYandong，HeAiwei，YuDaweiZhuYadong，LiFan，F(xiàn)engYu.

【Abstract】ObjectiveTo optimize the method for determining alkaloids in Sophora ammosa，and to determine thecontentof mainalkaloidsin Sophoraammosa byhigh-performance liquidchromatography （HPLC）. MethodsUsing C18 column，methanol and sodium phosphate bufer salt （PH8）as mobile phase，detection wavelength 210nm ，column temperature 35°C ，flow rate as chromatographic conditions，respectively， the content of 3 alkaloidsandsamples were determined，and thestandard curveand RSDof the method were evaluated.ResultsThe linear relationshipof the method wasgood inthestandard curve rangeof alkaloid concentration from O.54 to 60.00μg/ml ，and the absolute values of correlation coefficients were all greater than 0.992.The concentrations of low，medium and high alkaloids （6.O，12.O and 30.0μg/ml ）weredetermined.The average recovery ranged from 84.4% to 97.4% . The content of oxymatrine was 2.644mg/g ，sophorine 0.095mg/g and matrine was 0.047mg/g .Conclusion The optimised HPLC method can detect the content of 3 alkaloids in thesamples，which hastheadvantagesofhigh eficiencyandsaving time，andissuitable for the detection of alkaloidsin themulberryfruit.

【Key words】 Alkaloids； Mulberry fruit； High performance liquid chromatography

黑熱病是利什曼原蟲引起的病媒寄生蟲病，由雌性白蛉在哺乳動物宿主之間傳播1。（剩余5064字）