大理地區(qū)呼吸道病原菌核酸檢測(cè)結(jié)果分析

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中圖分類號(hào)：R446 文獻(xiàn)標(biāo)志碼：A DOI：10.3969/j.issn.1003-1383.2025.04.003

Analysis of nucleic acid detection results of respiratory tract pathogens in Dali area

DUAN Fuhui， LI Siying， YANG Xitong，WANG Guangming （Gene Testing Center，theFirst Affiliated Hospitalof Dali University，Dali 671OoO，Yunnan，China）

【Abstract】ObjectiveToanalyze the distributionof pathogenic bacteria inlowerrespiratory tractinfection in Dali area， soas toprovidediagnosticbasis for clinicallowerrespiratorytractinfection，and guideclinicalrationaluseofantibiotics.

MethodsAtotalof5O39samplesofrespiratorytractpathogenscollctedfromDaliresidentsintheFirstAffliated Hospital ofDaliUniversityfromNovember2O21toFebruary2O23wereanalyzed，andlop-mediated isothermalamplification （LAMP）wasused to detect respiratory tract pathogens andanalyze their distribution.ResultsOverallpositive rate was 45. 68 % （2302/5039），among which the top5infections were Haemophilus influenzae，Streptococus pneumoniae，Mycoplasma pneumoniae，Staphylococcus aureus and Klebsiella pneumoniae.Themain infection type was singlepathogenic bacteria infection，andthetop3wereHaemophilus influenzae，Mycoplasma pneumoniaeandStreptococus pneumoniae.Theoveralpositive detection rate in adults （ 43.03% ）was significantly lower than that in children（7 2.11% ）. The pathogen detection rate in males was higher than that in females，and difference was statistically significant（ χ2=6.483 P=0.011 ）. Conclusion LAMPcanquicklyandaccuratelydetectthepathogens ofrespiratorytractinfection.InDaliarea，the most widelydistributed pathogen inlowerrespiratorytractinfectionsisHaemophilus influenzae.Theoverallpositiverateofinfectioninmalesishigherthanthat infemales，andthepositivedetectionrate inchildrenis higherthanthatinadults.Theinfectiontype ismainly dominated by single-pathogen infection.

【Keywords】lower respiratory tract infection；isothermal nucleicacidamplification chip method；pathogen distribution; rational use of antimicrobial agents

呼吸道感染是臨床最常見的感染性疾病之一，由病原體侵入呼吸道從而繁殖致病，分為上呼吸道感染和下呼吸道感染[1]。（剩余6067字）