小麥莖基腐病相關(guān)基因TaDIR-B1的分子檢測(cè)及遺傳效應(yīng)分析

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Molecular Detection and Genetic Effect Analysis of TaDIR-B1 Gene Associated with Fusarium Crown Rot in Wheat

Zhao Qi1 ，Guo Can2，Cheng Dungong3，Li Faji3，Li Haosheng3 （1. College of Life Sciences of Yantai University， Yantai 264006， China; 2. Shangqiu Academy of Agriculture and Forestry Sciences ， Shangqiu 4760oo， China; 3. Crop Research Institute， Shandong Academy of Agricultural Sciences/National Engineering Research Center of Wheat and Maize/Key Laboratory of Wheat Biology and Genetics and Breeding in Northern Huang-Huai River Plain，Ministry of Agriculture and Rural Affairs/Shandong Technology Inovation Center of Wheat/ Jinan Key Laboratory of Wheat Genetic Improvement， Jinan 2501OO， China）

AbstractFusarium crown rot （FCR） has become one of the most important diseases in the main wheat production region of China.Detecting the distribution of FCR resistance alelesand analyzing their genetic effcts could provide guidance and available resources for wheat FCR resistance breeding.TaDIR-Bl gene has been reported to be involved in the regulation of wheat lignin synthesis，whose los-of-function could significantly improve lignin synthesis，and then improve the resistance to FCR.In the present study，a total of 106 wheat varieties （lines） originated from home and abroad were used as materials，the disease severity was investigatd by inoculation test with FCR in field，and the distributionof diferent variants and their genetic effects on FCR were analyzed by TaDIR-Bl sequencing. The results showed that among the 1O6 materials，73 belonged to mutant type （TaDIR-B1b ）which accounting for 68.87% of the total，and 33 belonged to wild type （TaDIR-Bla）which accounting for 31.13% of the total. The mean disease severity of FCR in the materials carrying TaDIR-B1b was lower than that in the materials carrying TaDIR–BIa ，and the differences in three environments and the mean value were significant （ P<0.05 ）or extremely significant. The proportion of TaDIR-B1b materials in Shandong and Hebei Province was relatively higher.Jimai 17O3，Mantol，Baimangmai， Shi 4185，Boomer and Azulon carried TaDIR-Bla gene and had lower disease severity of FCR，which could be used as parents for resistant variety breeding.

KeywordsWheat; Fusarium crown rot; Genetic effect; Molecular marker

小麥?zhǔn)俏覈?guó)第三大糧食作物，其高產(chǎn)穩(wěn)產(chǎn)對(duì)保障我國(guó)糧食安全具有重要現(xiàn)實(shí)意義。（剩余6190字）