哈茨木霉T9131鑒定及其拮抗病原菌和誘導(dǎo)黃芪抗病的作用分析
打開文本圖片集
中圖分類號：Q945.8 文獻(xiàn)標(biāo)識碼：A 文章編號：1000-3142（2025）08-1508-10
Abstract：Thefungus Trichodermaspp.is ubiquitouslydetectableinsoil andplantrots，and it playsacrucial roleinthe biologicalcontrolof agriculture.To explore the influenceof Trichoderma spp.ontheresistanceof Astragalus membranaceus to rotrot，thisstudyisolatedandidentifiedTrichodermaspp.fromAstragalus membranaceusrootrot，andsubsequently investigated itsantagonisticactiononpathogenandanalyzed itsefectsonphysiological indexes of diseaseresistanceof Astragalus membranaceus，determined the typeof Trichodermaspp.throughmorphological characteristics，ITSand tef1 sequenceanalysis，analyzedantagonistic effects of Trichoderma spp.isolate on Fusarium solani HYFS-1 byplate confrontationassy.Thisstudyalso determined thephysiological indexessuch ascatalase（CAT），superoxidedismutase （SOD），phenylalanine ammonia-lyase（PAL），peroxidase（POD）and proline（Pro）to clarify the roleof Trichoderma spp.isolate ininducingthediseaseresistanceof Astragalusmembranaceus.Theresults wereasfollows：（1）TheTrichoderma spptype isolated from Astragalus membranaceus root rotwas identifiedas Trichoderma harzianumandnamed T9131.（2） Theinhibition rate of Trichoderma harzianum T9l31 against Fusarium solani HYFS-1 reached 72%±1% after6days of confrontation.（3）Compared to HYFS-1alone，T9131significantlyenhanced SODactivityat0hafter HYFS-1infection；at the 24 h HYFS-1 infection，T9131markedlyincreasedSODactivity，PODactivity，andProcontent;bythe48hHYFS-1 infection，T9131significantlyelevatedPODactivity，PALactivity，andProcontent.Tosumup，Trichodermaharziaum T9131 cancontrolAstragalus membranaceus rotrotbydirectlyinhibitingthe growthof HYFS-1andinducingtheactivities of SOD，POD，PALandProcontentof Astragalus membranaceus under Fusarium solani HFS-1 stres.Thisresearch will laythefoundationforexploring thebiocontroleffectofTrichodermaharzianumanditsmachanismofresistanceofAstragalus membranaceus root rot.
Keywords：Astragalus membranaceusroot rot，Trichoderma harzianum，F(xiàn)usarium solani，antagonistic action， physiological index determination
黃芪（Astragalusmembranaceus）是豆科多年生草本植物，分為膜莢黃芪（A.membranaceus）或蒙古黃芪（A.membranaceusvar.mongholicus）兩大類，以根入藥，是藥用價值很高的中藥材（任小霞等，2016）。（剩余14443字）