熱形態(tài)建成中HY5抑制SAUR1/2/3/4的轉(zhuǎn)錄

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中圖分類號：Q943 文獻標識碼：A 文章編號：1000-3142（2025）08-1496-12

Abstract：The SAUR（SMALL AUXIN UP RNA） gene family is an important downstream gene family that promotes hypocotyl elongation.However，molecularmechanism by which SAURsareregulatedin high temperature-induced hypocotylelongationremainsunclear.Thermomorphogenesis isdefinedasaseriesof morphological changes thatoccur in a mildhigh temperature range between theoptimal temperature and the adverse high temperature in higher plants.Hightemperatured-induced hypocotylelongationisoneofthemostintensivelyresearchedphenomena.Inthisstudy， Arabidopsis Col wild type，hy5 mutant，35S：： HY5-HA/Col overexpressed plants and tobacco were usedasresearch materials.InhibitorNPA（N-1-naphthylphthalamicacid）treatment，quantitativeRT-PCR，chromatin immunoprecipitation and double luciferase reporter gene assay were used to explore the molecular mechanisms of high temperature regulation of SAUR1/2/3/4. The results wereas follows：（1）Auxin was downstream of HY5 （ELONGATED HYPOCOTYL5） in the thermomorphogenesis signaling pathway.（2） HY5 inhibited the transcription of SAUR1/2/3/4at 20 C and 29 C .（3）At normal temperature and high temperature，HY5 bound to the E-boxcontainig regions of SAURl/2/3/4 promoter chromatin，and these bindings were inhibited by high temperature.（4） Auxin wasrequired fortheregulation of SAUR1/2/3/4 by HY5.In summary，high temperatureregulates the transcriptionof thesefour genesbyaffecting the binding strengthof HY5toSAUR1/2/3/4promoterchromatin，and this regulatory process requires auxin.This study provides new insights into the molecular mechanisms of hypocotyl elongation downstream genes regulated by high temperature.

Key words： thermomorphogenesis，hightemperature-iduced hpocotyl elongation，HY5，AURs，transcriptionglation

隨著全球變暖的持續(xù)加劇，高溫將日益嚴重地危害全球植物的生存繁衍乃至農(nóng)作物生產(chǎn)（Challinor et al.，2014；Cox etal.，2020）。（剩余15320字）