缺血性中風患者舌苔非靶向脂質代謝組學研究

打開文本圖片集

中圖分類號：R277.7 文獻標志碼：A 文章編號：1007-3213（2025）05-1060-06

DOI：10.13359/j.cnki.gzxbtcm.2025.05.003

Non-targeted Lipid Metabolomics of Tongue Coating in Patients with IschemicStroke

LI Cuicui，CAI Donghao，XU Jingwen，LI Hui （Guangdong Second Traditional Chinese Medicine Hospital，Guangzhou 51Oo95 Guangdong，China）

Abstract：Objective To explore the diferences of lipid metabolites in tongue coating between ischemic stroke patients and healthy people，thus to screenout the potential biomarkers for tongue coating of ischemic stroke patients.Methods Twenty patients confirmed as ischemic stroke were selected as the observation group，and another 2O controls receiving healthy medicalcheckup atthesame period wereselected as the control group.Allof them were aged between 40 and 8O years. Using ultra-high performance liquid chromatography-masspectrometry （UHPLC-MS） technology，and by combined with the statistical methods of principal component analysis （PCA） and partial least squares discriminant analysis （PLS-DA），non-targeted lipid metabolomics study was performed on tongue coating samples of the observation group and the control group.According to the criteria that the VIP value in the orthogonal signal correction-based orthogonal partial least squares discriminant analysis（OPLS-DA） model was greater than 1 and the P value of the Wilcoxon rank test was less than O.O5，lipid metabolites with significant diferences werescreenedanddetermined.Results Differential lipid metabolitesexist inischemic stroke patients（observation group）and healthy people（control group），and a total of 14 lipid metabolites were identified，and they were free fatty acids，ceramide，sphingomyelin，cardiolipin，lecithin，etc.Of the 14 lipid metabolites，4 were down-regulated and 1O were up-regulated，and the diferences were statisticall significant （ P<0.05 ）.Conclusion There exist diferential lipid metabolites in tongue coating of patients with ischemic stroke and the healthy controls，and these different lipid metabolites are expected to become potential biomarkers for ischemic stroke.

Keywords：ischemic stroke；tongue coating；lipid metabolites；non-targeted lipid metabolomics；ultra highperformance liquid chromatography-mass spectrometry technology；UHPLC-MS；potential biomarkers

缺血性中風（ischemicstroke，IS）是最常見的腦血管疾病，中醫(yī)表現(xiàn)為氣虛血行無力，瘀血阻于經絡，是血證的一種I，具有高發(fā)病率、高致殘率、高死亡率的特點。（剩余8122字）