DNA分子鑒定和理化分析相結(jié)合對靈芝質(zhì)量評價(jià)的研究與應(yīng)用

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關(guān)鍵詞：靈芝；DNA分子鑒定；理化分析；質(zhì)量評價(jià)

中圖分類號(hào)：R282.5 文獻(xiàn)標(biāo)志碼：A 文章編號(hào)：1007-3213（2025）04-0988-04

DOI：10.13359/j.cnki.gzxbtcm.2025.04.027

Research and Application of Quality Evaluation of Ganoderma lucidum Based on the Combination of DNA Molecular Identification and Physicochemical Analysis

ZHANG Qingying，DONG Yanyan （Liaocheng Vocational and Technical College，Liaocheng 252Ooo Shandong，China）

Abstract：Objective The quality evaluation of 2O batchesof Ganoderma wascariedout bythe combinationof DNA identification and physicochemical analysis.Methods The 20 batches of Ganoderma were identified by DNA extraction，polymerase chain reaction （PCR）amplification，purification，sequencing，and inputing the Chinese medicines into DNA barcode identification system.Then the content determination of marker components was carried out.Results Ganoderma of No.1，2，3，4，5，6，9，10，11，12，13，14，15，16，18 and 20 belonged to Ganoderma lucidum（Leyssex Fr.）Karst.in the Pharmacopoeia，while No.7，8，17and 19 did notbelong to Ganoderma lucidum （LeyssexFr.）Karst.in thePharmacopoeia.Contentdetermination was conducted on16 batches of Ganoderma lucidum （Leyssex Fr.） Karst.， in which 15 batches were up to the standards of Pharmacopoeia，the decreasing sequence of contents was as follws：No.2，1，5，10，18，3，4，16，15， 11，13，14，6，9，and 12，and No.2O was failed to meet the standards，with a content of 0.38% . Conclusion The combination of DNA molecular identificationand physicochemical analysis can beused for rapid quality evaluation of the authenticity and superiority of Ganoderma.

Keywords： Ganoderma；DNA molecular identification；physicochemical analysis；quality evaluation

靈芝是利用最早和文化最厚重的食藥用菌之一，其市場需求日益增長，已知靈芝種類有88種2、質(zhì)量參差不齊，嚴(yán)重制約了其臨床應(yīng)用的廣泛性和有效性。（剩余4471字）