紅景天苷通過下調(diào)HMGB1、自噬抑制肝癌細胞增殖和侵襲

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關(guān)鍵詞：紅景天苷；肝癌；HMGB1；自噬；增殖；侵襲；H22細胞

中圖分類號：R285.5 文獻標志碼：A 文章編號：1007-3213（2025）04-0982-06

DOI：10.13359/j.cnki.gzxbtcm.2025.04.026

Salidroside Inhibits Proliferation and Invasion of Hepatocellular Carcinoma Cells Through Down-regulating HMGB1 and Autophagy

YANGLimin'，LI Shanlin2，WANGJin1 （1.Dept.of Gastroenterology，TheFirstAfiliated Hospitalof Zhengzhou University，Zhengzhou450052Henan，China; 2.Dept.of Gastroenterology，Zhoukou Central Hospital，Zhoukou 466O99 Henan， China）

Abstract：Objective To observe the mechanism of anti-hepatocellular carcinoma of salidroside.Methods H22 hepatocellular carcinoma cells were divided into control group（only fresh culture medium was replaced）and salidroside low ，medium- and high dose groups，and the corresponding intervention was given in each group for 48 hours. Cellular proliferation activity was detected by cell counting kit（CCK） -8 ，the number of invasive cells was detected by Transwell assay，the number of autophagosomes was observed by using dansylcadaverine （MDC）staining method，the mRNA and protein expression levels of high mobility group protein 1（HMGB1）and autophagy-related genes yeast Atg6 homolog（Beclin1），microtubule-associated protein1A/1B-light chain 3 （LC3 II/I）and sequestosome 62（p62） were detected accordingly byreal-time quantitative polymerasechain reaction （qRT-PCR）and Western Blot，respectively.Results Compared with the control group，the cellular proliferation activity was decreased，the numberof invading celsand autophagosomes were reduced insalidrosidelow-，mediumand high-dose groups，mRNA and protein expresion level and of p62 were increased，and mRNA and protein expressionlevels of HMGB1，LC3-II/I and Beclin1 weredecreased，the diferences being statistically significant （ P<0.05 ），showing a dose-dependent manner. Conclusion Salidroside may inhibit the proliferation and invasion of hepatocellular carcinoma cels by down-regulating the expression of HMGB1 and autophagy level.

Keywords：salidroside；hepatocelular carcinoma；HMGB1；autophagy；proliferation； invasion；H22 cells

肝癌具有隱匿性、轉(zhuǎn)移早、進展快以及侵襲性強等發(fā)病特點，發(fā)病率呈逐年上升趨勢，目前，手術(shù)切除、肝移植和化療是臨床治療肝癌的主要手段，但是，仍存在耐藥性、預(yù)后不良以及5年生存率低等情況，因此，尋求新的有效治療肝癌的方法具有重要意義。（剩余8595字）