KLF4在子癇前期患者胎盤(pán)及血清中的表達(dá)及臨床意義

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Expressionandclinical significance ofKLF4 inplacenta and serum ofpatients withpreeclampsia

TANG Yi 1，2 ，MENGQ i1，3 ，XUYunzhao1，2 （Department of Obstetricsand Gynecology，Afiliated Hospital ofNantong University，Jiangsu ; 2Medical School of Nantong University; 3Dalian Medical University）

[Abstract]Objective：To explore therelationshipbetween Kruppel-like factor 4（KLF4）andthe developmentof preclampsia（PE），anditsclinical significance.Methods：Atotalof68patients withPEwereselected，including37cases of early-onset preeclampsia （EPE） （EPE group）and 31 cases of late-onset preclampsia （LPE）（LPE group）.Sixty normal pregnant women inthe third trimester of pregnancy were taken as theNP group.Theimmunohistochemistryand protein bloting method wereused todetect the expresionof KLF4in placentaltissues;theenzyme-linked immunosorbentassay （ELISA）wasusedtodetectthelevelofKLF4inperipheralbloodandumbilicalarteryblood，andPearsoncorrelationtest wasused to analyse the relationship between serum KLF4 level inthe patients with PEandthequantitative amount of 24 h urineprotein.Results：TherelativeexpressionlevelofKLF4 protein inplacentaltissuesof thePE group wasO.61±0.10， which waslower than O.98±0.11 of the NP group（ P <0.05）; the relative expression level of KLF4 protein in placental tissues of the EPE group was 0.34±0.09，which was lower than 0.67±0.12 of the LPE group（P<0.05）.The concentration of KLF4 in the peripheral'serum of the PE group was 1.78±0.09 ，which was lower than 1.85±0.08 of the NP group（ P< 0.05）；theconcentration of KLF4intheumbilicalarteryserum of thePE groupwas1.56±O.13，which was lower than 1.66±0.08 of the NP group （P<0.01） ； the correlation analysis showed a negative correlation between the level of KLF4 in the PE group and the quantitative amount of 24 h urine protein （r=-0.7150， P <0.001）.Conclusions： The expression of KLF4 isdecreased inplacental tisues，peripheralbloodandumbilicalartery bloodof PEpatients.Thelevelof KLF4 in peripheral serum isnegativelycorrelated withthe24hurine proteincontent，suggesting thatthelowexpressionofKLF4is related to the occurrence and development of preeclampsia.

[Key Words]preeclampsia；Kruppel-likefactor4；immunohistochemistry；protein bloting;enzyme-linked immunosorbent assay

子癇前期（preeclampsia，PE）是妊娠期婦女獨(dú)有的復(fù)雜產(chǎn)科并發(fā)癥，具有異質(zhì)性、多系統(tǒng)性、進(jìn)行性等特點(diǎn)。（剩余5026字）