β-細辛醚對腎癌增殖及遷移侵襲的影響及其分子機制

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Effects and mechanism of β-asarone on inhibiting cell proliferation/migration/invasion of renal cell carcinoma

ZHANG Pan1，MEI Yibo1，HE Dalin2，ZENG Jin2

（1.The First Clinical Medical College of Xi’an Jiaotong University Health Science Center；2.Department of Urology，The First Affiliated Hospital of Xi’an Jiaotong University，Xi’an 710061，China）

ABSTRACT：Objective

To investigate the effects and molecular mechanism of β-asarone （β-As） on renal cell carcinoma （RCC）.Methods  Human RCC 786-O and 769-P cells were used in in vitro experiments，and murine RCC Renca cells were used in in vivo experiments.MTT，wound healing assay and Transwell assay were used to evaluate cell migration and invasion.Western blot was used to detect changes in protein levels during autophagy and

epithelial-mesenchymal transition （EMT）.Xenograft models of Balb/c mice were used to detect the anti-tumor effect of β-As in vivo.Results  β-As inhibited cell growth in a concentration-dependent manner;upregulated the expression of E-cadherin，but down-regulated the expressions of N-cadherin and Vimentin；inhibited cell proliferation，migration and invasion.β-As upregulated the expression of LC3 and p-AMPK，downregulated the expressions of p-mTOR and p-S6K in a concentration-dependent manner，but had no effects on the expressions of AMPK and mTOR.β-As inhibited the proliferation of RCC cells in vivo.Conclusion  β-As inhibits the proliferation，migration，invasion and EMT of RCC cells through the autophagy-dependent AMPK/mTOR signaling pathway，which may provide new ideas for the treatment of RCC，especially advanced RCC.

KEY WORDS：renal cell carcinoma；β-asarone；autophagy；migration；invasion；epithelial-mesenchymal transition；AMPK；mTOR

摘要：

目的  研究β-細辛醚（β-As）對腎癌細胞的抑制作用及其分子機制，幫助闡明β-As抗腎腫瘤的作用并為其臨床轉化提供參考。（剩余15423字）