不同檢驗(yàn)方法在乙型肝炎血清標(biāo)志物檢測(cè)中的應(yīng)用

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Applicationof different test methods in detecting serum markers of hepatitis B Yuan Songyan.Department of Clinical Laboratory，JinshuiDistrict General Hospital，Zhengzhou 45oooo，China

【Abstract】 ObjectiveTo explore the application value of electrochemiluminescence immunoassay in serological markersof hepatitisB.Methods12OsuspectedhepatitisBpatients，allof whom were treatedin JinshuiGeneralHospitalofZhengzhou fromAugust2O21 toSeptember2O23，wereexaminedby electroluminescenceimmunoassay，colloidal gold immunochromatography，enzyme-linkedimmunoassayand fluorescence quantitative polymerase chain reaction（PCR）.The resultsof the fluorescence quantitative PCR test weretakenasthe"gold standard"of diagnosisandanalyzed.Thepositiveratesof diferent detectionmethods in serum markers of hepatitis B （HBV surface antigen （HBsAg），Hepatitis Be antigen （HBeAg），hepatitis B surface antibody （anti-HBS），HepatitisBeantibody （anti-HBE）and HepatitisBcore antibody （anti-HBC） wereanalyzed. The diagnostic value of diferent detection methods in HBsAg was comparedand the consistency was calculated. ResultsOf the 12O suspected hepatitis B patients，44 cases were positive and76 cases were negative by quantitative PCR.There wasno significant diference in thepositive detectionrates of HBsAg，HBeAg，anti-HBS， anti-HBE andanti -HBC among diferentdetectionmethods（P>0.O5）.The diagnostic sensitivity，specificity， accuracy，and positive and negative predictive values of electroluminescence immunoassay in HBsAg were higher than thoseofcolloidal gold immunochromatographyandenzyme-linked immunoassay，with statistical diferences（ P< 0.05）.The consistency testshows thatcoloid-gold immunochromatography was not in good agreement with the "gold standard" （kappa value=0.366， P <0.001），ELISA was in good agreement （kappa value=0.472， P <0.001）， and electrochemical luminescence immunoassay was in good agreement （kappa value=0.859， P <0.001）. Conclusion Diferent detectionmethods havecertain clinicalvalue in theapplicationof hepatitis B serum markers，each with advantagesand disadvantages，but electrochemical luminescenceimmunoassay has high applicationvalue，high diagnostic accuracyand strong consistency，which can provide certain reference basis for the diagnosisand treatment of hepatitisB，and hashigh clinical application value.

【Key Words】Electrochemical luminescence immunoassay；Coloidal gold immunochromatography；Enzymelinked immunoassay; Hepatitis B surface antigen; Accuracy

乙型肝炎（以下簡(jiǎn)稱“乙肝”為傳染性疾病的一種，可經(jīng)母嬰傳播、性傳播、血液傳播等，對(duì)人類身體健康威脅較大，且若患者得不到及時(shí)有效的治療，隨著病情發(fā)展，可致患者死亡，預(yù)后效果較差[2]。（剩余6071字）